Selection of protocols we frequently use:
ChIP, RT-qPCR and RNA-seq
Live-cell imaging
Ubiquitin pulldown and protein extracts
- Ni-Ub pulldown under denat. conditions
- Protein extraction w/ NaOH/TCA
- Preparation of chromatin-enriched fraction
- NuPage protein gel casting protocol
Large-scale protein purification of chromatin-associated proteins
Cloning & PCR
- Sequence- & ligation-independent cloning (SLIC)
- Primer design for KOs, tagging and qPCR
- Perl script for KO primers (coming soon)
Yeast methods
- Yeast transformation
- Pombe transformation
- Random spore analysis
- Colony PCR w zymolyase
- Yeast DNA prep (‘smash & grab’)
- large-scale genomic DNA preparation
- Yeast plating assays with pin-frogger (serial dilutions)
SGA with Singer RoToR HDA