lem2_KO_for	lem2	for	CATTGCTCCGCCCGGTTTAGAATT
lem2_KO_rev	lem2	rev	GGGTTCGACACTATGGATGCCTAT
lem2_Chk_for	lem2	for	CGACATCGCTGGAAGCAACCATTG
lem2_Chk_rev	lem2	rev	GTCCGTTCTTTCTCGGCTTAATCC
lem2_WT_int_for	lem2	for	AAAGCGTCTAGCTCCGAGGATGAA
lem2_WT_int_rev	lem2	rev	GAAGAGGACTAGAAGTGGCTGAAG


This is the information on oligos for lem2 
	The coordinates, ORF and DNA strand of lem2 are:
	2234598-2236664	SPAC18G6.10	Watson
	The size of non-spliced lem2 is 2067 bp

	The start codon is *** ATG *** and 
	the total length of the saved sequence file 
	(incl. add. 900 bp at 5' and 3'UTR) is 3867 bp. 

The amplified kanMX cassette is 2552 bp.
The amplified corresponding WT sequence is 3275 bp.

lem2 Chk for	 annT 57 degree and 54% GC	rel.pos is -601
lem2 KO for	 annT 55 degree and 50% GC	rel.pos is -582
lem2 KO rev	 annT 55 degree and 50% GC	rel.pos is +487
lem2 Chk rev	 annT 55 degree and 50% GC	rel.pos is +626
lem2 WT int for	 annT 55 degree and 50% GC	rel.pos is 213
lem2 WT int rev	 annT 55 degree and 50% GC	 rel.pos is 643