An easy way to save time, tips, the 96-well plates, and increase SYBR stability:
There are now three new RT-qPCR programs in the Integra Vialab folder for the respective steps (SYBR, primers, sample)
What's new:
- In each program: the target plate is the combination of the cooling block with the 384-well plate (you simply place the 384-well plate on top of the cooling block, as shown)
- Primer program: source plate for the primers is no longer the 96-well plate but instead the tube-rack (shown in the picture)
A few things to keep in mind:
-> The three programs serve as
template and would need to be adapted according to your qPCR layout
->
Volume of primers in tube rack: You can use as little at 120 μL of1.5 μM primers 1.5ml tubes for 24 wells. (The robot can even pick up as little as 35 μL which will work for a couple wells)
-> If your qPCR layout doesn't allow use of the tube rack for your primers, using the cooling block is still a good idea for retaining SYBR's stability throughout the pipetting process
-> cooling block is very cold when taking it from the -20 but doesn't stay cold for the entirety of a set up
Why now:
Prior to having the cooling block there were issues with the robot using the 8-channel purple pipette with the tube rack. Now, we no longer need to pipette primers into a plate and can keep them in the tubes. We use less tips for the transfer and you can easily store your left over primers after use.
