Selection of protocols & methods we frequently use

Cloning & PCR

• Sequence- & ligation-independent cloning (SLIC)
• Primer design for KOs, tagging and qPCR
• Perl script for KO primers (coming soon)

Yeast methods

• Yeast transformation
• Pombe transformation
• Random spore analysis
• Colony PCR w zymolyase
• Yeast DNA prep (‘smash & grab’)
• large-scale genomic DNA preparation
• Yeast plating assays with pin-frogger (serial dilutions)

SGA with Singer RoToR HDA

• RoToR SGA protocol for S. pombe
• Plates & pads cleaning